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You are here:Home » Antibodies » Abs to Antibiotics » Anti -Chloramphenicol

Anti -Chloramphenicol

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E IF
Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma, and Chlamydia. It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit. This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds. It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia. Chloramphenicol has other adverse effects such as bone marrow depression, and gray baby syndrome. Itís use is limited to serious infections such as those where resistance to other antibiotics occurs. It is still widely used in the treatment of typhoid fever, meningitis and eye infections.
Catalog #C4350-02
ApplicationsSuitable for use in ELISA and Fluoroimmunoassay (FIA). Other applications have not been tested.
Recommended DilutionIndirect ELISA: 1:2000; Coat with 400ng of chloramphenicol-BSA per well.
FIA (Polarization Fluoroimmunoassay): 1:750; Tracer is aromatic amino derivative of chloramphenicol labeled by reaction with fluorescein isothiocyanate (10nmol/L).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
Concentration~10-12mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by ammonium sulfate frationation.
ImmunogenAromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) coupled to KLH, using the diazotization procedure.
SpecificityRecognizes Chloramphenicol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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