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Chloramphenicol Acetyltransferase (CAT) Recombinant
Chloramphenicol Acetyltransferase (CAT) Recombinant
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| Chloramphenicol Acetyltransferase (CAT) is suitable for use in cell extract assays. CAT, encoded by Tn9, catalyzes the transfer of an acetyl group from acetyl-CoA to the 3’-hydroxy position of chloramphenicol. | | | Catalog # | C4350-10 | | Source | Recombinant E. coli | | Purity | 95% (SDS-PAGE) | | Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.8, 50uM 2-mercaptoethanol, 50uM chloramphenicol, 50% glycerol. | | Activity | ~10-14 units/ul | | Unit Definition | One unit is defined as the amount of enzyme required to transfer 1nmol of acetyl-CoA or butyryl-CoA to chloramphenicol in one minute at 37°C. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | 9040-07-7 | | Molecular Weight | 22-24kD | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | E. coli |
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