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Cholesterol, Cholesteryl Ester, BioAssay™ Quantitation Kit
Cholesterol, Cholesteryl Ester, BioAssay™ Quantitation Kit
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| The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H2O2, which reacts with a sensitive cholesterol probe to produce color ( max = 570nm) and fluorescence (Ex/Em = 535/587nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase, or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters). | | | Catalog # | C5045-15 | | Kit Components | Cholesterol Assay Buffer, 1x25ml | | Cholesterol Probe (in DMSO, anhydrous), 1x200ul, Ready to use as supplied. Warm to room temperature to thaw the DMSO solution. Store at –20°C, protect from light. Use within two months | | Enzyme Mix (lyophilized), 1x1vial, Dissolve in 220ul Cholesterol Assay Buffer before use. Aliquot and store at –20°C. Use within two months. | | Cholesterol Esterase (lyophilized), 1x1vial, Dissolve in 220ul Cholesterol Assay Buffer before use. Aliquot and store at –20°C. Use within two months. | | Cholesterol Standard (2mg/ml), 1x100ul | | Storage and Stability | Store kit at –20°C, protect from light. Allow Assay Buffer and Cholesterol Probe to warm to RT before use. Keep enzymes and cholesterol standard on ice while using. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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