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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Cholesterol, Cholesteryl Ester, BioAssay™ Quantitation Kit II

Cholesterol, Cholesteryl Ester, BioAssay™ Quantitation Kit II

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Specifications

Cholesterol is an essential molecule in all animal life. It has been involved in both normal
development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple
method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a
colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These
esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free
cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
Catalog #C5045-18
Kit ComponentsCholesterol Assay Buffer 1x25ml
Substrate Mix (lyophilized) 1x1vial
Enzyme Mix (lyophilized) 1x1vial
Esterase (lyophilized) 1x1vial
Cholesterol Standard (2ug/ul) 1x100ul.
Storage and StabilityStore at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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