| Acyl-Coenzyme A is cholesterol acyltransferase-2 (ACAT-2) which catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kD.5 Detects ACAT-2 at about 60 kD in murine liver, intestine, and fibroblasts.6 |
|
| Catalog # | C5045-38 |
| Applications | Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunocytochemistry: 1:500, 1us/ml |
| Immunohistochemistry: 1:500, 1ug/ml |
| Western Blot: 1:250, 2ug/ml for 1 hour at RT. Overnight incubations at 4ºC with greater dilutions can produce optimal results. |
| Optimal dilution to be determined by researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in TBS, pH 7.4, with 0.1% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa 3-20 (P3GGARLRLQRTEGLGGER20) of human ACAT-2 |
| Specificity | Recognizes human ACAT-2. Species Crossreactivity: Mouse, rat, sheep and porcine. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
