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You are here:Home » Antibodies » Abs to Enzymes, Acyltransferase » Anti -Cholesterol Acyltransferase 2 (Acyl Coenzyme A Cholesterol Acyltransferase 2, ACAT2, Sterol O-Acyltransferase 2)

Anti -Cholesterol Acyltransferase 2 (Acyl Coenzyme A Cholesterol Acyltransferase 2, ACAT2,
Sterol O-Acyltransferase 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Acyl-Coenzyme A is cholesterol acyltransferase-2 (ACAT-2) which catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kD.5 Detects ACAT-2 at about 60 kD in murine liver, intestine, and fibroblasts.6
Catalog #C5045-38
ApplicationsSuitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunocytochemistry: 1:500, 1us/ml
Immunohistochemistry: 1:500, 1ug/ml
Western Blot: 1:250, 2ug/ml for 1 hour at RT. Overnight incubations at 4ºC with greater dilutions can produce optimal results.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.4, with 0.1% BSA, 0.02% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 3-20 (P3GGARLRLQRTEGLGGER20) of human ACAT-2
SpecificityRecognizes human ACAT-2. Species Crossreactivity: Mouse, rat, sheep and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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