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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -Cholesterol Oxidase (CHOD) (Biotin)

Anti -Cholesterol Oxidase (CHOD) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology. Cholesterol esterase catalyzes the hydrolysis of sterol esters into their component sterols and fatty acids. The enzyme is found primarily in the pancreas, but has been detected in other tissues as well. Bile salts, such as cholate and its conjugates, are required to stabilize the enzyme in its native polymeric form and to protect it from proteolytic hydrolysis in the intestine (Vahouny and Treadwell 1968). Cholesterol esterase finds clinical applications in the determination of serum cholesterol (Allain et al. 1974). Cholesterol esterase is synthesized in the acinar cells of the pancreas, stored in zymogen granules, and secreted as a component of pancreatic juice into the lumen of the small intestine (Labow et al. 1983). Cholesterol esterase hydrolyzes a wide range of ester substrates including cholesteryl esters, acylglycerides, phospholipids (Brockerhoff and Jensen 1974), retinyl esters (Fredrikzon et al. 1978), vitamin esters, and phenyl esters (Rudd and Brockman 1984). The enzyme has also been found to have amidase activity (Hui et al. 1993). The enzyme is useful as a biocatalyst because of its ability to catalyze transacylation reactions in a water-limited environment (Gallo et al. 1977, Kazlauskas 1989, and Feaster et al. 1996). Cholesterol esterase is a glycoprotein that in the presence of salts aggregates to a hexamer (Hyun et al. 1971). Cholesterol esterase belongs to the alpha/beta-hydrolase fold family (Ollis et al. 1992, Cygler et al. 1993). Most members of this family are esterases and share secondary and tertiary features. Nearly all use a serine esterase catalytic mechanism, which resembles that of the serine proteases (Kraut 1977). The gene that encodes cholesterol esterase in pigs (lipA) is located on chromosome 14 (GENE ID: 100142668). The LIPA gene is conserved in human, chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C. elegans, S. pombe, S. cerevisiae, E. gossypii, M. grisea, N. cassa, A. thaliana, and rice.
Catalog #C5048-07B
ApplicationsSuitable for use in ELISA, Western Blot, Immunoelectrophoresis, Single Radial Immunodiffusion and Inhibition. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified by ammonium sulfate precipitation and Ion Exchange chromatography.
ImmunogenCholesterol oxidase isolated and purified from Nocardia erythropolis
SpecificityRecognzies cholesterol oxidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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