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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Choline, Acetylcholine BioAssay™ Kit

Choline, Acetylcholine BioAssay™ Kit

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Specifications

Choline and acetylcholine play important roles in many biological processes. This Choline/Acetylcholine Assay Kit is good for 100 assays and provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color ( = 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol to~5 nmol of choline or acetylcholine.
Catalog #C5052-01
Kit Summary• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit can detect 10 pmol to ~5 nmol of choline or acetylcholine.
Kit ComponentsC5052-01A: Choline Assay Buffer, 1x25ml
C5052-01B: Choline Probe, 1x200ul. Ready to use. (red cap)
C5052-01C: Choline Enzyme Mix, 1x1vial (green cap)
C5052-01D: Acetylcholinesterase, 1x1vial (blue cap)
C5052-01E: Choline Standard (5umol), 1x1vial (yellow cap)
Storage and StabilityStore all components at -20°C. Shelf life of three months after receipt. Protect C5052-01B (Choline Probe) from light and moisture (use within two months). For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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