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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH

Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Catalog #	C5057
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 10-20ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q38
Host	Mouse
Source	Porcine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified
Immunogen	Highly purified ChAT obtained from porcine brain
Specificity	Recognizes porcine choline acetyltransferase (ChAT)-positive cells. Species Crossreactivity: human and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.