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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)

Anti -Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline
O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetylase, aka choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker
Catalog #C5057-02E
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Positive ControlHuman fetal brain tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to the C-terminus of human Choline acetylase (GST-tagged).
SpecificityRecognizes human Choline acetylase. Species Crossreactivity: rat, mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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