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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (ChAT)

Anti -Choline Acetyltransferase (ChAT)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified IH IC
Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71.721 kD protein (640aa) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.
Catalog #C5057-03
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:1000 -1:2000
Immunocytochemistry: 1:1000 -1:2000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to Choline Acetyltransferase (KLH). Species sequence homology: human (P28329, NCBI) and mouse (Q03059, NCBI) 100%.
SpecificityRecognizes Choline Acetyltransferase. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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