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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (CHAT)

Anti -Choline Acetyltransferase (CHAT)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum B IH
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Catalog #C5057-06
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with no preservatives added.
PuritySerum
ImmunogenHuman placental enzyme.
SpecificityRecognizes cholinergic neurons in human brain and central nervous systems. Species Crossreactivity: Mouse, guinea pig and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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