Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (CHAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl CoA:choline O-acetyltransferase)

Anti -Choline Acetyltransferase (CHAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl
CoA:choline O-acetyltransferase)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #C5057-06A
Alternate Name CHAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl CoA:choline O-acetyltransferase
Domain Information
Gene Ontology Terms
Application(s)Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kD band observed in Human Placenta lysates (calculated MW of 70.5kD according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3ug/ml.
0.03ug/ml staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C5057-06A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenCKEKATRPSQGHQP, C Terminus
SpecificityThis antibody is expected to recognize both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1. Species Crossreactivity: Human, chimpanzee, porcine, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links