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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (CHAT)

Anti -Choline Acetyltransferase (CHAT)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH IC
Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein(640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.
Catalog #C5057-06B
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:250 -1:500
Immunohistochemistry: 1:100 -1:200
Immunocytochemistry: 1:100 -1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 10mM; isotonic 0.9%, with 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P_066264, NCBI) and mouse (Q03059,NCBI) sequences.
SpecificityRecognizes human CHAT. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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