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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (CHAT)

Anti -Choline Acetyltransferase (CHAT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Catalog #C5057-08
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:2000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconsitute with 100ul sterile ddH2O.
PuritySerum
ImmunogenA synthetic peptide corresponding to 22aa H-GLFSSYRLPGHTQDTLVAQKSS from porcine ChAT, conjugated to KLH
SpecificityRecognizes rat choline Acetyltransferase. Will stain cholinergic neurons in rat central and peripheral nervous systems. Other antibodies produced against the same peptide have shown to react against primate and human enzyme.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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