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You are here:Home » Antibodies » Abs to Enzymes, Acetyltransferase » Anti -Choline Acetyltransferase (CHAT)

Anti -Choline Acetyltransferase (CHAT)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites IH
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Catalog #C5057-09
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q28
HostMouse
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid.
PurityAscites
ImmunogenCholine acetyltransferase purified from rat brain
SpecificityRecognizes rat cholinergic neurons in the brain and central nervous system. Reported to crossreact with chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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