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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Cholinergic Receptor, Nicotinic, alpha 7, Exons 5-10 (CHRFAM7A, CHRNA7 and FAM7A Family with Sequence Similarity 7A, Exons A-E Fusion, CHRNA7, CHRNA7-DR1, CHRNA7-FAM7A Fusion Protein, D-10, MGC120482, MGC120483)

Anti -Cholinergic Receptor, Nicotinic, alpha 7, Exons 5-10 (CHRFAM7A, CHRNA7 and FAM7A
Family with Sequence Similarity 7A, Exons A-E Fusion, CHRNA7, CHRNA7-DR1, CHRNA7-FAM7A
Fusion Protein, D-10, MGC120482, MGC120483)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed.
Catalog #C5062-74
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA:1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Brain (Amygdala, Hippocampus, Cerebellum) lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C5062-74-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, QKYCIYQHFQFQ, from the N-Terminus of human CHRFAM7A.
SpecificityRecognizes human CHRFAM7A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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