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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH RIA

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Catalog #	C5067-01
Applications	Suitable for use in ELISA, RIA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:400-1:3200
	RIA: 1:50-1:6400
	Western Blot: 1:1000-1:4000 (ECL)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	2Q552
Host	Mouse
Source	Mouse
Concentration	Not Determined
Form	Supplied as a liquid.
Purity	Ascites
Immunogen	Mouse proteoglycan
Specificity	Recognizes both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin 4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase. Species Crossreactivity: bovine, sheep, canine and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.