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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E RIA

Catalog #	C5067-51
Applications	Suitable for use in ELISA and RIA. Other applications not tested.
Recommended Dilutions	ELISA: 1:100-1:5000
	RIA: 1:100-1:200
	Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q551
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, no preservative added.
Purity	Ascites
Immunogen	Chondroitinase ABC-digested adult human aggrecan.
Specificity	Recognizes human Chondroitin 6 Sulfate. Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. Does not react with native proteoglycans, dermatan sulfate, keratan sulfate or chondroitin-4-sulfate. Species Crossreactivity: chondroitin-6-sulfate stubs of all proteoglycans of all species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.