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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Chondroitin 6 Sulfate

Anti -Chondroitin 6 Sulfate

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E RIA
Catalog #C5067-51
ApplicationsSuitable for use in ELISA and RIA. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:5000
RIA: 1:100-1:200
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q551
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, no preservative added.
PurityAscites
ImmunogenChondroitinase ABC-digested adult human aggrecan.
SpecificityRecognizes human Chondroitin 6 Sulfate. Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. Does not react with native proteoglycans, dermatan sulfate, keratan sulfate or chondroitin-4-sulfate. Species Crossreactivity: chondroitin-6-sulfate stubs of all proteoglycans of all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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