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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Chondroitin Sulfate Proteoglycan (NG2, CSPG)

Anti -Chondroitin Sulfate Proteoglycan (NG2, CSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC IF
The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors1. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias2. Studies indicate that NG2 is involved in modulating cellular response to growth factors 3-5 and cell motility 6-7; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries8. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration 8-9. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis10. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors11.
Catalog #C5067-70
ApplicationsSuitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Other applications not tested.
Recommended DilutionImmunofluorescence Microscopy: Cells in culture
Immunoprecipitation: 1ug/10e6 cells
FACS analysis: 1-2ug/10e6 cells
Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q550
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.25M sodium chloride, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.
SpecificityRecognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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