| NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor. |
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| Catalog # | C5067-71 |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.25-0.5ug/ml. Detects NG2 in a whole rat brain preparation. Whole rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with C5067-71. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunohistochemistry: 1ug/ml; Reported to immunostain 4% paraformaldehyde fixed adult rat brain sections. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5H26 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | HEK293 cells expressing a truncated integral membrane form of NG2 consisting of aa1592-2222. |
| Specificity | Recognizes rat NG2, Mr ~270-300kD. |
| Species Sequence Homology | Mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
