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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Chondroitin Sulfate Proteoglycan (NG2, CSPG)

Anti -Chondroitin Sulfate Proteoglycan (NG2, CSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Catalog #C5067-71
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.25-0.5ug/ml detects NG2 in a whole rat brain preparation. Whole rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with C5067-71. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.
Immunohistochemistry: 1ug/ml is reported to immunostain 4% paraformaldehyde fixed adult rat brain sections.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt x Mo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5H26
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1592-2222 of Chondroitin Sulfate Proteoglycan expressed as a truncated integral membrane form in HEK293 cells. Species Sequence Homology: mouse
SpecificityRecognizes rat Chondroitin Sulfate Proteoglycan, Mr ~270-300kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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