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Anti -Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)
Anti -Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like
Domain-containing Brain Protein, CSPG5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IC |
|
| Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein of 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa31-423) that contains a Ser117 CS attachment site and an EGF-like domain (aa 371-413). There are at least two isoform variants. One shows a deletion of aa 487-513, while a second shows an alternaive start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a >75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC. | | | Catalog # | C5067-91B | | Applications | Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other Applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Neuroglycan C is approximately 0.3ng/well. | | Western Blot: 1ug/ml | | Immunocytochemistry: 10ug/ml was used to detect Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT, After blocking, cells were incubated with diluted C5067-91B for 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a powder lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 0.5ml sterile PBS. | | Purity | Purified by immunoaffinity chromatography | | Immunogen | CHO cell-derived, recombinant human Neuroglycan C extracellular domain (aa 31 - 420; Accession # AAQ04776) | | Specificity | Recognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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