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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)

Anti -Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like
Domain-containing Brain Protein, CSPG5)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IC
Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein of 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa31-423) that contains a Ser117 CS attachment site and an EGF-like domain (aa 371-413). There are at least two isoform variants. One shows a deletion of aa 487-513, while a second shows an alternaive start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a >75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC.
Catalog #C5067-91B
ApplicationsSuitable for use in ELISA, Immunocytochemistry and Western Blot. Other Applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml. The detection limit for recombinant human Neuroglycan C is approximately 0.3ng/well.
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml was used to detect Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT, After blocking, cells were incubated with diluted C5067-91B for 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a powder lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 0.5ml sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenCHO cell-derived, recombinant human Neuroglycan C extracellular domain (aa 31 - 420; Accession # AAQ04776)
SpecificityRecognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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