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Anti -Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)
Anti -Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like
Domain-containing Brain Protein, CSPG5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IC |
|
| Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein oft 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa 31-423) that contains a Ser117 CS attachment site and an EGF-like domain aa 371-413). There are at least two isoform variants. One shows a deletion of aa87-513, while a second shows an alternative start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a > 75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC. | | | Catalog # | C5067-91C | | Applications | Suitable for Immunohistochemistry ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1ug/ml | | Western Blot: 1ug/ml | | Immunohistochemistry: 10ug/ml detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked | | with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RTfollowed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | ~0.2mg/ml | | Form | Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml. | | Purity | Purified by mouse Neuroglycan C affinity chromatography. | | | Immunogen | Purified, CHO cell-derived, recombinant mouse Neuroglycan C (rmNeuroglycan C; aa 31-420; Accession# AAH55736). | | Specificity | Recognizes Neuroglycan C, extracellular domain. In direct ELISA, this antibody shows 25% cross-reactivity with rhNeuroglycan C. Species Crossreactivity: Mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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