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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Chorionic Gonadotropin, Human (hCG)

Chorionic Gonadotropin, Human (hCG)

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Specifications

Human Chorionic Gonadotropin (hCG) is a peptide hormone produced in pregnancy, that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testing generally is based on the detection or measurement of hCG.
Catalog #C5069-21
Human Chorionic Gonadotropin is produced from a sterile preparation of placental glucoprotein urine of pregnant women having a total molecular mass of 36,700 Daltons. The hCG consists of 237 amino acids, a chain-92 amino acids and b chain-145 amino acids. The hCG is purified by proprietary chromatographic techniques.
Biological Activity~5000IU/mg
ContaminantsFree of HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of hCG as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after rexeipt. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight36.7kD
SourceUrine of pregnant women
Purity98%, purified by proprietary chromatographic techniques
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O to 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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