| Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma. |
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| Catalog # | C5069-77 |
| Applications | Suitable for use in RIA, ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-04D IgG, H&L (AP) Pab Gt xMo |
| I1904-06D IgG, H&L, X-Adsorbed (AP) Pab Gt xMo |
| I1904-04A: IgG, H&L (FITC) Pab Gt xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H479 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.09% sodium azide. |
| Purity | Purified by Ion Exchange chromatography. |
| Immunogen | Highly purified Chorionic Gonadotropin |
| Specificity | Recognizes free human chorionic gonadotrophin beta (hCG beta). Does not bind to holo-hGG, holo-hLH or free hLH beta. Recognizes the beta 7 epitope on hCG beta. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
