Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Subunit A, CHAF1A, CAF1B, Chromatin Assembly Factor I p150 Subunit, CAF-I 150kD Subunit, CAF1P150, CAF-I p150, hp150, MGC71229, P150)

Anti -Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit
A, CAF-1 Subunit A, CHAF1A, CAF1B, Chromatin Assembly Factor I p150 Subunit, CAF-I 150kD
Subunit, CAF1P150, CAF-I p150, hp150, MGC71229, P150)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP IF
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly
of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple
chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).
Catalog #C5069-94C
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence (IF-IC): 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1020
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A.
SpecificityRecognizes endogenous levels of total CHAF1A. Species Crossreactivity: monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesChromatin Assembly Factor 1, Subunit A (p150)


External Links