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You are here:Home » Antibodies » Abs to Chromogranin » Anti -Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Anti -Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1,
Pituitary secretory protein I, Vasostatin, SPI)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Chromogranin A, a protein of 439-amino acid which is encoded on chromosome 14, is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Catalog #C5069-97
ApplicationsSuitable for use in Immunohistocemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: 1-2ug/ml at RT for 2 hours.
Optimal dilutions to be determined by the researcher.
Positive controlPC-12 cells. Pancreas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.218
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See C5069-97X.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman pheochromocytoma. Cellular Localization: Cytoplasmic
MW of antigen68-75kD.
SpecificityRecognizes human Chromogranin A at 68-75kD. Species Crosseactivity: monkey, porcine, rat and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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