| Chromogranin A (CgA) is an 86kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and |
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| inhibit hormone and neurotransmitter release (1). Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor (TNF ) induced gap formation in human umbilical venous endothelial cells (2). |
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| CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients (3). |
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| Catalog # | C5069-97M |
| Applications | Suitable for use inWestern Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1,000,000 |
| Immunohistochemistry: 1:250-500 |
| Immunoprecipitation:1:30 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9E223 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of human Chromogranin A. |
| Specificity | Recognizes human Chromogranin A. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
