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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC FC

Chromogranin A (CgA) is an 86kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release (1). Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor (TNF ) induced gap formation in human umbilical venous endothelial cells (2). CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients (3).
Catalog #	C5069-97Q
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10,000-50,000
	Immunohistochemistry: 1:100-250
	Immunocytochemistry: 1:100-250
	Flow Cytometry: 1:20
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E224
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues near the N-terminus of human Chromogranin A
Specificity	Recognizes human Chromogranin A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.