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You are here:Home » Antibodies » Abs to Chromogranin » Anti -Chromogranin A (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Anti -Chromogranin A (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory
protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Chromogranin A is a protein of 439aa which is encoded on chromosome 14. It is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Catalog #C5069-97X
ApplicationsSuitable for use in Immunohistocemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot: 1-2ug/ml for 2 hours at RT
Optimal dilutions to be determined by the researcher.
Positive control PC-12 cells, Pancreas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.218
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See #C5069-97.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman pheochromocytoma. Cellular Localization: Cytoplasmic
SpecificityRecognizes human Chromogranin A at 68-75kD.
Species CrossreactivityMonkey, porcine, rat and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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