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You are here:Home » Antibodies » Abs to Chromogranin » Anti -Chromogranin A, mixed clones (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Anti -Chromogranin A, mixed clones (BSA & Azide Free) (CGA, CHG A, Pancreastatin,
Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Catalog #C5069-98X
ApplicationsSuitable for use in Immunohistochemistry (formalin/paraffin). Other applications not tested.
Recommended DilutionsImmunohistochemistry: 0.5-1.0ug/ml for 30 min at RT. Although not required, staining of formalin/paraffin tissues is ENHANCED by boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Positive ControlPancreas
Cellular LocalizationCytoplasmic
MW of Antigen68-75kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F131
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See Cat # C5069-98.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman pheochromocytoma
SpecificityThe cocktail is especially designed for sensitive detection of Chromogranin A in formalin-fixed, paraffin-embedded tissues. Species Crossreactivity: human and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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