Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-CNTF » Ciliary Neurotropic Factor, Recombinant, Rat (CNTF)

Ciliary Neurotropic Factor, Recombinant, Rat (CNTF)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Ciliary Neutrophic Factor (CNTF) is a potent neural factor originally characterized as a survival factor for chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.
Catalog #C5073
Recombinant rat CNTF is a 22.7kD protein containing 199aa residues, lacking a hydrophobic N-terminal signal for secretion.
Biological ActivityDetermined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 25-35ng/ml.
Amino Acid SequenceAFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight22.7kD
SourceE. coli
Purity~98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links