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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Catalog #	C5073-05
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA (Indirect): 0.5-2ug/ml. Use 100ul/well antibody solution. When used in conjunction with compatible secondary reagents, 0.2-0.4ng/well of recombinant hCNT can be detected.
	ELISA (Sandwich): 0.5-2ug/ml. Use 100ul/well antibody solution. When used in conjunction with C5073-05A as a detection antibody, 0.2-0.4ng/well of recombinant hCNTF
	can be detected.
	Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit is 1.5-3ng/lane, under reducing or non-reducing conditions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Highly purified (>98%), human recombinant Ciliary Neurotropic Factor (hCNTF)
Specificity	Recognizes human Ciliary Neurotropic Factor (CNTF)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.