Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested.

ELISA: 0.5ug-2ug/ml Detects 0.2-0.4ng/well of recombinant rat CNTF (using 100ul/well antibody solution). Neutralization: 0.02-0.05ug/ml Yields one-half maximal inhibition [ND50] of the biological activity of rat CNTF (0.15ng/ml). Western Blot: 0.1-0.2ug/ml detects recombinant rat CNTF at 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant rat CNTF.

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul ddH2O.

Purified by immunoaffinity chromatography.

Recognizes rat ciliary neurotrophic factor (CNTF).