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You are here:Home » Molecular Biology » Growth Factors-CNTF » Ciliary Neurotrophic Factor, Recombinant, Human (CNTF)

Ciliary Neurotrophic Factor, Recombinant, Human (CNTF)

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Specifications

Human ciliary neutotrophic factor (CTNF), is a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
Catalog #C5073-05H
SourceRecombinant corresponding to human CTNF, 199aa, expressed in E. coli.
Molecular Weight~22.7kD
Endotoxin 1EU/ug
ActivityThe ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
ApplicationsSuitable for use in Functional Assays. Other applications not tested.
Recommended DilutionFunctional Assays: 50ng/ml-150ng/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 200ul of 5mM sodium phosphate, pH 7.5. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRecombinant, expressed in E. coli
PurityPurified: ~98% (SDS PAGE/HPLC)
FormSupplied as a lyophilized powder in 5mM sodium phosphate, 75mM sodium chloride. Preservative free. Reconstitute with 200ul of 5mM sodium phosphate, pH 7.5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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