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Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)
Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)
Pricing
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| Catalog # | C5073-06J | | Source | Recombinant corresponding to 199aa of human Ciliary Neurotrophic Factor expressed in E.coli is a single, non-glycosylated polypeptide chain. | | Molecular Weight | ~22.7kD | | Biological Activity | The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 5x10 IU/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~22.7kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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