Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » Growth Factors-CNTF » Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)

Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Catalog #C5073-06J
SourceRecombinant corresponding to 199aa of human Ciliary Neurotrophic Factor expressed in E.coli is a single, non-glycosylated polypeptide chain.
Molecular Weight~22.7kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 5x10 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~22.7kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links