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Anti -Ciliary Neurotrophic Factor (CNTF)
Anti -Ciliary Neurotrophic Factor (CNTF)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B N |
|
| Catalog # | C5073-09D | | Applications | Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA (Indirect, Sandwich): 0.5ug-2ug/ml Detects 0.2-0.4ng/well of recombinant rat CNTF (using 100ul/well antibody solution). | | Neutralization: 0.02-0.05ug/ml Yields one-half maximal inhibition [ND50] of the biological activity of rat CNTF (0.15ng/ml). | | Western Blot: 0.1-0.2ug/ml Detects recombinant rat CNTF at 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | | | | Country of Origin: | | USA | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly pure (> 98%) recombinant rat CNTF. | | Specificity | Recognizes rat CNTF. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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