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You are here:Home » Antibodies » Antibodies-Neurotrophins » Anti -Ciliary Neurotrophic Factor (CNTF)

Anti -Ciliary Neurotrophic Factor (CNTF)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #C5073-09D
ApplicationsSuitable for use in ELISA, Neutralization and Western Blot. Other applications not tested.
Recommended DilutionELISA (Indirect, Sandwich): 0.5ug-2ug/ml Detects 0.2-0.4ng/well of recombinant rat CNTF (using 100ul/well antibody solution).
Neutralization: 0.02-0.05ug/ml Yields one-half maximal inhibition [ND50] of the biological activity of rat CNTF (0.15ng/ml).
Western Blot: 0.1-0.2ug/ml Detects recombinant rat CNTF at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Country of Origin:
Clone TypePolyclonal
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant rat CNTF.
SpecificityRecognizes rat CNTF.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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