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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Circulating Immune Complexes (CIC) BioAssay™ ELISA Kit

Circulating Immune Complexes (CIC) BioAssay™ ELISA Kit

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Specifications

Catalog #C5090
Intended UseThis Circulating Immune Complexes (CIC) BioAssay™ ELISA Kit kit has been specially developed to measure CIC in mouse serum.
Principle of the TestThe CIC ELISA test is based on the binding of CIC (complement fixing type) to C1q coated on microtiter plates. Diluted serum samples are incubated with the coated plates. After a washing step, anti-mouse IgG (HRP) conjugate is added. After another washing step, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of CIC present in the sample. The reaction is terminated by adding stopping solution. Absorbance is read at 450nm. Concentration of CIC in samples can be determined by comparing with positive controls or standards.
To confirm the presence of CIC, serum samples can be diluted in regular sample diluent and in “Confirmation Solution” (provided in the kit) that is known to disrupt binding of CIC to C1q. Both samples are then measured at the same time. A decrease of CIC by at least 30% in “Confirmation Solution” confirms the presence of CIC in samples.
Kit ComponentsC5090A: Microtiter Plate, 1x96 wells (12 strips). Coated with purified C1q.
C5090B: Negative Control (10X), 1x100ul.
C5090C: Positive Control (10X), 1x100ul.
C5090D: Sample Diluent (20X), 1x10ml. Supplied as a red-colored liquid. Dilute 1:10 with distilled water.
C5090E. IgG (HRP) (100X), 1x150ul. Dilute 1:100 with 1X Sample Diluent before use.
C5090F: Confirmatory Solution, 1x10ml. Supplied as a green-colored liquid.
C5090G. Wash Buffer (100X), 1x10ml. Dilute 1:100 with distilled water before use.
C5090H: TMB Substrate, 1x12ml.
C5090J: Stop Solution, 1x12ml.
Storage and StabilityAll kit components are stable at 4°C. The whole kit stability is 6 months after receipt. The unused strips should be stored tightly covered with adhesive film and with the desiccant in the bag.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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