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You are here:Home » Antibodies » Abs to Enzymes, Synthase » Anti -Citrate Synthase

Anti -Citrate Synthase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the Citric Acid Cycle (or Krebs Cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.
Citrate synthase catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the six-carbon citrate. Oxaloacetate will be regenerated after the completion of one round of the Krebs Cycle.
Catalog #C5700
ApplicationsSuitable for use in ELISA, Western Blot, Dot Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsDot Blot: Non-reducing conditions only
Immunohistochemistry: Formaldehyde-fixed, paraffin embedded sections
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No2Q2258
HostMouse
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified
ImmunogenPorcine heart citrate synthase.
SpecificityRecognizes porcine citrate synthase. Shows intense intracellular granular reactivity with human muscle and liver when tested by IHC on formaldehyde-fixed, paraffin embedded tissue sections. In the case of anaerobic damage/necrosis, the affected cells showed characteristic loss of reactivity compared to normal tissue.
Species CrossreactivityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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