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Specifications

Citric acid (HOOC-CH2-C(-OH)(-COOH) -CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.
Catalog #	C5802
	Citrate BioAssay™ Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (lmax W0nm) and fluorescent (Ex/Em, 535/587nm) product. The Citrate BioAssay™ Kit can detect 0.1-10nmoles (~2uM-10mM) of citrate in a variety of samples.
Kit Components	C5802A: Assay Buffer, 1x25ml
	C5802B: Probe, 1 vial
	C5802C: DMSO, 1x400ul
	C5802D: Enzyme Mix, 1 vial
	C5802E: Developer, 1 vial
	C5802F: Standard (10umol), 1 vial
Storage and Stability	Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.