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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E IH

Citrulline exists primarily in the liver, where it is involved in the urea cycle to detoxify and excrete ammonia. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine. In bacteria, the arginine dihydrolase pathway deaminates arginine to citrulline and splits citrulline into ornithine and carbamoyl phosphate. This pathway is a source of energy for many microorganisms and is used as an identifying test. Citrulline is not a component of any major proteins or enzymes.
Catalog #	C5806
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:1000-1:5000.
	Optimal dilutions to be determined by the researcher.
Crossreactivity	Using a conjugate Citrulline-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
	Compound Crossreactivity ratio (a)
	Citrulline-G-BSA 1
	Arginine-G-BSA >1:100,000
	Glutamine-G-BSA >1:100,000
	Ornithine-G-BSA >1:100,000
(a)	Citrulline-G-BSA concentration/unconjugated or other conjugated concentration at half displacement
	G = Glutaraldehyde, BSA = Bovine Serum Albumin
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid. No preservative added.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic DL.Citrulline (BSA).
Specificity	Recognizes Citrulline.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.