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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -c-Jun

Anti -c-Jun

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IC
Catalog #C5815
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000 detected c-Jun in RIPA lysates from human A431 cells, and previously in Jurkat cell lysate. A431 RIPA cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C5815 (1:1000ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ul
Immunocytochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Positive Control A0001-50
Non-stimulated A431 cell lysate. Use 10-20ug per lane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
PurityNeat serum
ImmunogenTrp E fusion-protein containing the DNA binding domain of avian c-Jun.
SpecificityRecognizes avian c-Jun at ~ 35kD. Species crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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