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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).
Catalog #	C5815-03B
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:2,000
	Immunohistochemistry: 1:250
	Immunocytochemistry: 1:250
	Immunoprecipitation: 1:40
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k129
Host	Rabbit
Source	Human
Form	Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide from N-terminus of human c-Jun.
Specificity	Recognizes human c-Jun. Species crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.