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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -c-Jun, phosphorylated, Ser63

Anti -c-Jun, phosphorylated, Ser63

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).
Catalog #C5815-03C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:5,000-10,000
Immunohistochemistry: 1:50-100
Immunocytochemistry: 1:100-200
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k130
HostRabbit
SourceHuman
FormSupplied as a liquid
PuritySupernatant
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Ser63 of human c-Jun
SpecificityRecognizes human c-Jun phosphorylated at Ser63.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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