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You are here:Home » Molecular Biology » MB-Transcription Factors » c-Jun, phosphorylated (Ser63) Control Peptide

c-Jun, phosphorylated (Ser63) Control Peptide

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Specifications

c-Jun is a member of the Jun Family composed of c-Jun, JunB and JunD, and is a component of
the transcription factor AP-1. AP-1 is composed dimers of Fos, Jun and ATF family members, and binds and activates transcription at TRE/AP-1 elements (1). Extracellular signals including growth factors, chemokines and extracellualar stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues
and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and the context of the cell (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as promising therapeutic target
for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).
Catalog #C5815-10A
Corresponding antibody, C5815-10, detects endogenous levels of c-Jun only when phosphorylated at Ser63. Does not recognize the phosphorylated forms of JunD or JunB. Species Crossreactivity Mouse and rat.
ApplicationsSuitable for use as a blocking peptide to evaluate the specificity of antibody reactivity in immunohistochemistry and Western blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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