| Total c-Jun Sandwich ELISA Antibody Pair is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and a HRP-Conjugated Secondary Antibody (1000X stock) is supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-Conjugated Secondary Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein. |
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| Catalog # | C5815-10D |
| A. Kit Components | Total c-Jun Capture Antibody (100X), 1x400ul |
| Total c-Jun Detection Antibody (100X), 1x4000ul |
| Anti-mouse IgG (HRP) (1000X), 1x40ul |
| B. Reagents Required But Not Supplied | 1. Coating Buffer: 1X PBS, (20X PBS) 3.2 mM Na 2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, pH 7.4 |
| 2. Wash Buffer: 1X PBS/0.05% Tween-20, (20X PBST) |
| 3. Blocking Buffer: 1X PBS/0.05% Tween-20, 1% BSA |
| 4. 1X Cell Lysis Buffer: (10X Cell Lysis Buffer) 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM ethylene diamine tetraacetate (EDTA), 1 mM ethylene glycol-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA), 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM |
| Na3VO4, 1 g/ml leupeptin. |
| 5. TMB Substrate: (TMB Substrate) |
| 6. STOP Solution: (STOP Solution) |
| NOTE: Reagents should be made fresh daily. |
| C. Storage and Stability Store Total c-Jun capture and detection antibodies at 4°C. Store Anti-mouse IgG (HRP) at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
