c-Jun, Total, ELISA Kit (Antibodies Only)
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| The Total c-Jun ELISA Kit (Antibodies Only) is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit (C5815-10H). Capture and Detection antibodies (100X stocks) and a HRP-Conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun capture antibody in PBS is coated overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun detection antibody and HRP-Conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein. | | | Catalog # | C5815-10G | | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (Reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun vival has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13). | | Species Crossreactivity Human, Mouse and Rat | | Kit Components C5815-10G1 | Total c-Jun Capture Antibody (100X), 1x400ul. Pink cap. | | C5815-10G2: Total c-Jun Detection Antibody (100X), 1x400ul. Blue cap. | | C5815-10G3: IgG-HRP Antibody (1000X), 1x40ul. Anti-mouse IgG conjugated to HRP. Yellow cap. | | Materials required but not supplied Phosphate Buffered Saline (PBS) Phosphate Buffered Saline (PBS) with Tween-20 TMB Substrate Stop Solution Blocking Buffer-PBS containing 0.05% Tween-20 and 1% BSA 96-well microplates (Corning® 96 Well Clear Polystyrene High Bind Stripwell™ Microplates (#2592) or Corning® 96 Well EIA/RIA Easy Wash™ Clear Flat Bottom Polystyrene High Bind Microplates (#3369)) Microplate reader | | Storage and Stability Store C5815-10G1 and C5815-10G2 at 4°C. Store C5815-10G3 at -20°C. Kit is stable for up to 6 months. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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