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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stressinduced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
Catalog #	C5815-11
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunofluorescence (IF-IC): 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A and immunoaffinity chromatography.
Immunogen	Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser73 of human c-Jun.
Specificity	Detects endogenous levels of c-Jun only when activated by phosphorylation at Ser73. Also recognizes phosphorylation of JunD at Ser100. Species Crossreactivity: Human, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.