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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified B

Catalog #	C5815-12E
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000 detects phospho-c-Jun in RIPA lysates from 3T3 cells that had been treated with anisomycin. Untreated and anisomycin treated 3T3 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho- c-Jun (1:500 dilution). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 85-101 (NGHITT[pT]PTPTQFLCPK) of human c-jun.
Specificity	Recognizes phospho-c-Jun, Mr 41kD. An unknown protein was also detected, Mr 52kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with rat and bovine based on sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.