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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -c-Jun, phosphorylated (Thr91, Thr93)

Anti -c-Jun, phosphorylated (Thr91, Thr93)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #C5815-14
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated c-Jun in RIPA lysates from 3T3 cells stimulated with 10ug/ml anisomycin for 30 minutes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to aa85-101 (NGHITT[pT]P[pT]PTQFLCPK) of human c-Jun. The immunizing sequence is identical in mouse and rat.
SpecificityRecognizes phosphorylated c-Jun (Thr91/Thr93), Mr 41kD. An additional unknown protein was also detected, Mr 65kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with avian, rat, rabbit, bovine and porcine based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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