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c-Jun (Ser63) II, c-Jun (Ser73) BioAssay™ Phosphorylation Detection Kit
c-Jun (Ser63) II, c-Jun (Ser73) BioAssay™ Phosphorylation Detection Kit
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| C-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements (Figure 1). The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73 (3, 4). The c-Jun (Ser63) II and c-Jun (Ser73) Antibody Kit provides reagents and protocols for the rapid analysis of c-Jun phosphorylation at Ser63 and at Ser73. The kit includes two phospho-antibodies that recognize c-Jun only when phosphorylated at Ser63 or Ser73, a c-Jun antibody (phosphorylationstate independent), protein controls and protein markers for western blots, and our HRP Western Detection System. | | | Catalog # | C5815-15 | | Applications | Suitable for use in Western Blotting, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western blotting: 1:1000 | | Immunocytochemistry: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Purity | Purified by protein A and peptide affinity chromatography. | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. | | Specificity | Detects phosphorylated Ser63 of c-Jun but does not appreciably crossreact with the corresponding phosphorylated form of JunD or JunB. Phospho-c-Jun (Ser73) antibody detects phosphorylated c-Jun at Ser73 or JunD at Ser100, yet will not react with up to 0.5ug of non-phosphorylated c-Jun or JunD. | | | Species crossreactivity | Human, mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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